CYP3A4 for compound screening and profiling via fluorescence-based cytochrome P450 inhibition assay quantification of enzyme activity.
Target Overview
CAT#
CYP3A4
Enzyme
CYP3A4+ NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP3A4 cDNA and rabbit Cytochrome P450 cDNA)
Synonym
Cytochrome P450, subfamily IIIA, polypeptide 4
Substrate
Fluorescence Substrate Vivid® BOMCC
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Ketoconazole | 4.07 |
| Proadifen (SKF-525A) | 0.28 |
| Aminobenzotriazole | 6.38 |
Scientific Information
P08684 (https://www.uniprot.org/uniprotkb/P08684/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP3A4
