AMPK (A1/B2/G3) Kinase Assay Service

Activity-based AMPK (A1/B2/G3) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

AMPK (A1/B2/G3)

Enzyme

Human AMPK (combination of A1/B2/G3 subunits)

HGNC

PRKAA1/PRKAB2/PRKAG3

Synonym

AMPK (A1/B2/G3)

Substrates

Peptide substrate, [HMRSAMSGLHLVKRR], 20 µM
AMP, 125 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
GW 5074	1,200
H-89	190
Ro 31-8220	58
Rottlerin	>100,000
Staurosporine	0.2

Scientific Information

Subunit A1: Q13131 (http://www.uniprot.org/uniprot/P49760)Subunit B2: O43741 (http://www.uniprot.org/uniprot/O43741)Subunit G3: Q9UGI9 (http://www.uniprot.org/uniprot/Q9UGI9)

Screening Location

Malvern, USA

Reference compound IC50 for AMPK (A1/B2/G3)