ARK5/NUAK1 Kinase Assay Service

Activity-based ARK5/NUAK1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

ARK5/NUAK1

Enzyme

Human ARK5/NUAK1

HGNC

NUAK1

Synonym

KIAA0537, ARK5

Substrates

Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

O60285 (http://www.uniprot.org/uniprot/O60285)

Screening Location

Malvern, USA

Reference compound IC50 for ARK5/NUAK1