c-Src (T341M) Kinase Assay Service

Activity-based c-Src (T341M) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

c-SRC (T341M)

Enzyme

Human c-SRC (T341M)

HGNC

SRC (T341M)

Synonym

ASV, SRC1, c-SRC, p60-Src

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
D-64406	6,200
Staurosporine	0.22

Scientific Information

P12931 (http://www.uniprot.org/uniprot/P12931)

Screening Location

Malvern, USA

Reference compound IC50 for c-SRC (T341M)