CDK8/cyclin C Kinase Assay Service

Activity-based CDK8/cyclin C kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

CDK8/Cyclin C

Enzyme

Human CDK8/Cyclin C

HGNC

CDK8/CCNC

Synonym

CDK8: Cyclin-dependent kinase 8, Cell division protein kinase 8, Protein Kinase K35
Cyclin C: CCNC

Scientific Information

CDK8: P49336 (https://www.uniprot.org/uniprot/P49336)
Cyclin C: P24863 (http://www.uniprot.org/uniprot/P24863)

Assay Properties

Substrates

Protein substrate, Pol2-CTD, 5 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 48
Ro 31-8220 960
GW5074 1,100
H-89 3,600
Rottlerin >100,000

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for CDK8/Cyclin C

Reference compound IC50 for CDK8/Cyclin C

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