Activity-based DGKA lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
DGKA
Enzyme
Human DGKA
HGNC
DGKA
Synonym
Diacylglycerol kinase alpha, DAG kinase alpha, DGK-alpha, DAGK1, DAGK
Scientific Information
P23743 (https://www.uniprot.org/uniprot/P23743)
Assay Properties
Substrates
DLG (1, 2-Dilauroyl-sn-glycerol), 500 µM + CaCl2, 2 mM
ATP, 50 µM
Reaction
1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+
Detection
ADP-Glo Assay
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| DGK-IN-1 (CAS No: 2407892-34-4) | 3.8 |
| Calphostin C | 1,038 |
| R-59-949 | 15,290 |
| R-59-022 | 86,160 |
| Cochlioquinone A | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for DGKA
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