ERK1/MAPK3 Kinase Assay Service

Activity-based ERK1/MAPK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ERK1

Enzyme
Human ERK1

HGNC
MAPK3

Synonym
ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 810
Ro 31-8220 8,500
GW5074 >20,000
SCH772984 2.3
LY3214996 0.3
Scientific Information
P27361 (http://www.uniprot.org/uniprot/P27361)

Screening Location
Malvern, USA

Reference compound IC50 for ERK1
Reference compound IC50 for ERK1

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