Activity-based ERN2/IRE2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ERN2/IRE2
Enzyme
Human ERN2/IRE2
HGNC
ERN2
Synonym
IRE2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
Staurosporine | 27 |
Ro 31-8220 | 2,700 |
GW5074 | 1,600 |
H-89 | 19,500 |
Rottlerin | >100,000 |
Scientific Information
Q76MJ5 (http://www.uniprot.org/uniprot/Q76MJ5)
Screening Location
Malvern, USA
Reference compound IC50 for ERN2/IRE2
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