ERN2/IRE2 Kinase Assay Service

Activity-based ERN2/IRE2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ERN2/IRE2

Enzyme
Human ERN2/IRE2

HGNC
ERN2

Synonym
IRE2

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 27
Ro 31-8220 2,700
GW5074 1,600
H-89 19,500
Rottlerin >100,000
Scientific Information
Q76MJ5 (http://www.uniprot.org/uniprot/Q76MJ5)

Screening Location
Malvern, USA

Reference compound IC50 for ERN2/IRE2
Reference compound IC50 for ERN2/IRE2