Activity-based FGFR1OP- FGFR1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR1OP- FGFR1
Enzyme
Human FGFR1OP- FGFR1 fusion
HGNC
CEP43-FGFR1
Synonym
FOP, CEP43
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
Staurosporine | 9.2 |
PP2 | 1,300 |
AG1478 | 8,100 |
PDGFR I-III | >100,000 |
D-64406 | 11,000 |
Scientific Information
Screening Location
Malvern, USA
Reference compound IC50 for FGFR1OP-FGFR1
![Reference compound IC50 for FGFR1OP-FGFR1](/wp-content/uploads/2023/11/FGFR1OP-_FGFR1_Kin_Malvern.png?itok=Vv19t6F9)
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