Activity-based FGFR2 (K641R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR2 (K641R)
Enzyme
Human FGFR2 (K641R)
HGNC
FGFR2 (K641R)
Synonym
BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, KGFR, KSAM, K-SAM, TK14, TK25
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3.1 |
| PP2 | 13 |
| AG1478 | 15 |
| PDGFR I-III | >50,000 |
| D-64406 | 210 |
Scientific Information
P21802 (http://www.uniprot.org/uniprot/P21802)
Screening Location
Malvern, USA
Reference compound IC50 for FGFR2 (K641R)
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