Activity-based MEKK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEKK1
Enzyme
Human MEKK1
HGNC
MAP3K1
Synonym
MAPKKK1, MEKK, MEKK1
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1,200 |
| Ro 31-8220 | >20,000 |
| GW 5074 | >20,000 |
| H-89 | >20,000 |
| Rottlerin | >20,000 |
Scientific Information
Q13233 (http://www.uniprot.org/uniprot/Q13233)
Screening Location
Malvern, USA
Reference compound IC50 for MEKK1
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