Activity-based MEKK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEKK2
Enzyme
Human MEKK2
HGNC
MAP3K2
Synonym
MAPKKK2, MEKK2B
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 15 |
| Ro 31-8220 | 330 |
| GW 5074 | 11,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q9Y2U5 (http://www.uniprot.org/uniprot/Q9Y2U5)
Screening Location
Malvern, USA
Reference compound IC50 for MEKK2
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