Activity-based MEKK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEKK3
Enzyme
Human MEKK3
HGNC
MAP3K3
Synonym
MAPKKK3, MEK kinase3
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 6.2 |
| Ro 31-8220 | 280 |
| GW 5074 | >10,000 |
| H-89 | >10,000 |
| Rottlerin | 23,600 |
Scientific Information
Q99759 (http://www.uniprot.org/uniprot/Q99759)
Screening Location
Malvern, USA
Reference compound IC50 for MEKK3
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