Activity-based PAK2 (Y443N) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PAK2 (Y443N)
Enzyme
Human PAK2 (Y443N)
HGNC
PAK2 (Y443N)
Synonym
Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, PAK-2, PAK65, Gamma-PAK, S6/H4 kinase
Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 7.1 |
| Ro 31-8220 | 11,500 |
| GW5074 | >100,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q13177 (http://www.uniprot.org/uniprot/Q13177)
Screening Location
Malvern, USA
PDF