Activity-based HIPK3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
HIPK3
Enzyme
Human HIPK3, wt
HGNC
HIPK3
Manning ID
HIPK3
Synonym
DYRK6, PKY, YAK1, HIPK3, ANPK, FIST, FIST3
UniProt
Q9H422 (HIPK3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
Compounds | IC50 (nM) |
---|---|
Staurosporine | 6200 |
Sorafenib | 6200 |
Sunitinb | 9400 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
FreeChoice | HIPK3 |
345 wt Profiler | Yes |
345 IC50 wt Profiler | Yes |
Mutant Profiler Addon | No |
Lipid Kinase Profiler Addon | No |
IC50 Profiler | No |
Recombinant Protein Availability
Active recombinant protein |
Yes; ProQinaseTM – HIPK3 |
Product Number | 0936-0000-1 |
Web Shop | Reaction Biology Online Shop |
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