Activity-based PI4KB kinase assay for compound screening and profiling via ADP-Glo – assay technology
Target Overview
RBE Target ID
PI4KB
Enzyme
Human PI4KB, wt
HGNC
PI4KB
Manning ID
N/A
Synonym
NPIK, pi4K92, PI4K92, PI4Kbeta, PI4KBETA, PI4K-beta, PI4K-BETA, PI4KIIIBETA, PIK4CB, PI4KB, PI4Kb
UniProt
Q9UBF8 (PI4KB_HUMAN)
Kinase Type
Lipid kinase
Kinase Group
PI3K
Assay Properties
Assay Format
ADP-Glo
Substrates
PI, ATP
Reaction
PI + ATP = P-PI + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Wortmannin | 8200 |
| LY294002 | 100000 |
| PI-103 | 43000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PI4KB |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | Yes |
| IC50 Profiler | IC50 Lipid Kinase Profiler |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – PI4KB |
| Product Number |
1157-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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