BRD4-2 NanoBRET TE Intracellular Assay

BRD4-2 NanoBRET Target Engagement Intracellular Assay for screening of compounds binding to the target, competitive binding assay format

Target Overview

Target

BRD4-2

HGNC Symbol

BRD4 (BD2)

Synonyms

HUNKI; MCAP; CAP; HUNK1

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET Intracellular Target Engagement Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-BRD4 (BD2) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET BRD Tracer and then treated with reference compound IBET-151 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target

Reference Compound IC50s

Compounds	IC50 (M)
IBET-151	2.257e-006

Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Cell-based Epigenetic Assays

Reference compound IC50 for BRD4 (BD2)

Reference compound IC50 for BRD4 BD2

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