HDAC6 CD2 NanoBRET Intracellular HDAC Assay

HDAC6 CD2 NanoBRET Target Engagement Intracellular HDAC Assay, competitive binding assay format

Target Overview

Target

HDAC6 CD2

HGNC Symbol

HDAC6 CD2

Synonyms

HDAC6 CD2, HDAC6 (CD2), HDAC6-2

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular HDAC Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-HDAC6 CD2 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET intracellular Target Engagement HDAC Tracer and then treated with reference compound SAHA for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target HDAC

Reference Compound IC50s

Compounds	IC50 (M)
SAHA	9.861e-008

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Cell-based Epigenetic Assays

Reference compound IC50 for HDAC6 CD2

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