Activity-based COMT (V108M) Methyltransferase Assay for compound screening and profiling via FlashPlate methyltranferase assay.
Target Overview
CAT#
COMT (V108M)
Enzyme
Human COMT (V108M)
Synonym
Catechol O-methyltransferase
Substrates
COMT-S01, 0.5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol [methyl-³H]
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Tolcapone | 1.1 |
| Entacapone | 0.6 |
| Ro 41-0960 | 0.6 |
Scientific Information
P21964 (http://www.uniprot.org/uniprot/P21964)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for COMT (V108M)
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