Activity-based DNMT3A Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
DNMT3A
Enzyme
Human DNMT3A
Synonym
DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA, DNM3A
Substrates
Lambda DNA, 0.0075 mg/ml
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + DNA = S-adenosyl-L-homocysteine + DNA 5-[methyl-³H]-cytosine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 271 |
| Sinefungin | 430 |
Scientific Information
Q9Y6K1 (http://www.uniprot.org/uniprot/Q9Y6K1)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for DNMT3A
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