Activity-based METTL21A Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
METTL21A
Enzyme
Human METTL21A
Synonym
Protein N-lysine methyltransferase METTL21A, HSPA lysine methyltransferase, HSPA-KMT, Hepatocellular carcinoma-associated antigen 557b, Methyltransferase-like protein 21A, FAM119A, HCA557B
Substrates
GST-HSPA8-[CTD], 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + protein L- lysine = S-adenosyl-L-homocysteine + protein [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 72,000 |
| Chaetocin | >100,000 |
| Sinefungin | >100,000 |
| Suramin | 6,200 |
Scientific Information
Q8WXB1 (http://www.uniprot.org/uniprot/Q8WXB1)
Screening Location
Malvern, USA
Reference compound IC50 for METTL21A
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