Activity-based SET8 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SET8
Enzyme
Human SET8
Synonym
Histone-lysine N-methyltransferase SETD8, H4-K20-HMTase SETD8, SET domain-containing protein 8, PR/SET domain-containing protein 07, Lysine N-methyltransferase 5A, KMT5A, PRSET7, SET07
Substrates
Nucleosomes, 0.05 mg/ml
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 110,000 |
| Sinefugin | 220,000 |
Scientific Information
Q9NQR1 (http://www.uniprot.org/uniprot/Q9NQR1)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for SET8
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