Activity-based SETD2 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SETD2
Enzyme
Human SETD2
Synonym
Histone-lysine N-methyltransferase SETD2, HIF-1, Huntingtin yeast partner B, Huntingtin-interacting protein 1, HIP-1, Huntingtin-interacting protein B, Lysine N-methyltransferase 3A, SET domain-containing protein 2, HYPB, KIAA1732, KMT3A, SET2
Substrates
Oligo Nucleosomes, 0.05 mg/ml
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 3,400 |
| Sinefugin | 81,400 |
| Chaetocin | 84 |
Scientific Information
Q9BYW2 (http://www.uniprot.org/uniprot/Q9BYW2)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for SETD2
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