Activity-based SMYD2 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SMYD2
Enzyme
Human SMYD2
Synonym
SET and MYND domain-containing protein 2, HSKM-B Lysine N-methyltransferase 3C, KMT3C
Substrates
Histone H3, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 526 |
| Sinefugin | 15,000 |
Scientific Information
Q9NRG4 (http://www.uniprot.org/uniprot/Q9NRG4)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for SMYD2
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