Activity-based SMYD3 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SMYD3
Enzyme
Human SMYD3
Synonym
Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, ZMYND1
Substrates
MEKK2, 0.5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + Protein L- lysine = S-adenosyl-L-homocysteine + Protein [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 17,000 |
| EPZ031686 | 3.5 |
| Sinefungin | >100,000 |
| Suramin | 4,800 |
Scientific Information
Q9H7B4 (http://www.uniprot.org/uniprot/Q9H7B4)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for SMYD3
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