Activity-based SUV39H1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SUV39H1
Enzyme
Human SUV39H1
Synonym
Histone-lysine N-methyltransferase SUV39H1, Suppressor of variegation 3-9 homolog 1, Su(var)3-9 homolog 1, Position-effect variegation 3-9 homolog, Histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, Lysine N-methyltransferase 1A, KMT1A, SUV39H, SUV91
Substrates
Histone H3, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 23,000 |
| Sinefungin | 140,000 |
| Chaetocin | 330 |
Scientific Information
O43463 (http://www.uniprot.org/uniprot/O43463)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for SUV39H1
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