METTL3/METTL14 Methyltransferase Assay Service

Activity-based METTL3/METTL14 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

METTL3/METTL14

Enzyme

Human METTL3/METTL14 Complex

Synonym

METTL3: Methyltransferase-like protein 3, N6-adenosine-methyltransferase 70 kDa subunit, MTA70

METTL14: Methyltransferase-like protein 14, N6-adenosine-methyltransferase non-catalytic subunit, KIAA1627

Substrates

METTL3/METTL14 RNA substrate, 0.5 µM
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-3H]methionine + adenosine in mRNA = S-adenosyl-L-homocysteine + N6-methyladenosine in mRNA [methyl-3H]

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 350
UZH1a 380
STM2457 4.2

Scientific Information

METTL3; Q86U44 (https://www.uniprot.org/uniprotkb/Q86U44)

METTL14; Q9HCE5 (https://www.uniprot.org/uniprotkb/Q9HCE5)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Methyltransferase Assays.

Reference compound IC50 for METTL3/METTL14

Reference compound IC50 for METTL3/METTL14

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