KDM4A Demethylase Assay for compound screening and profiling via HTRF-based quantification of enzyme activity.
Target Overview
CAT#
KDM4A
Enzyme
Human KDM4A
Synonym
Lysine-specific demethylase 4A, JMJD2A
Substrates
B-H3(21-44)-K36Me3, α-KG
Assay Properties
Reaction
H3(21-44)-K36Me3 + α-KG + O₂ = H3(21-44)-K36Me2 + succinate + CO₂ + formaldehyde
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| 2,4-PDCA | 138 |
| IOX1 | 2014 |
Scientific Information
O75164 (http://www.uniprot.org/uniprot/O75164)
Screening Location
Malvern, USA
Reference compound inhibition of KDM4A
PDF