Activity-based CBP Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.
Target Overview
CAT#
CBP
Enzyme
Human CBP
Synonyms
CREB-binding protein, CREBBP
Scientific Information
Q92793 (http://www.uniprot.org/uniprot/Q92793)
Assay Properties
Substrates
Histone H3, 5 μM
Acetyl CoA, 0.2 μM
Reaction
[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Anacardic Acid | 20,000 |
| Garcinol | 14,000 |
| Curcumin | 37,000 |
| C646 | 3,000 |
| PF-9363 | >10,000 |
| WM-8014 | >10,000 |
| WM-1119 | >10,000 |
| WM-3835 | >10,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Histone Acetyltransferase Assay (HAT) Services for Drug Discovery
Reference Compound IC50 for CBP
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