Activity-based KAT2A/GCN5 Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.
Target Overview
CAT#
KAT2A/GCN5
Enzyme
Human KAT2A/GCN5
Synonyms
Histone acetyltransferase KAT2A, Lysine acetyltransferase 2A Histone acetyltransferase GCN5, General control of amino acid synthesis protein 5-like 2, STAF97, GCN5L2, HGCN5, KAT2A
Scientific Information
Q92830 (http://www.uniprot.org/uniprot/Q92830)
Assay Properties
Substrates
Histone H3, 2.5 μM
Acetyl CoA, 0.2 μM
Reaction
[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| WM-8014 | >10,000 |
| WM-3835 | >10,000 |
| PF-9363 | >10,000 |
| WM-1119 | >10,000 |
| Anacardic Acid | 19,500 |
| Garcinol | 64,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Histone Acetyltransferase Assay (HAT) Services for Drug Discovery
Reference Compound IC50 for KAT2A/GCN5
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