Activity-based KAT5 Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.
Target Overview
CAT#
KAT5
Enzyme
Human KAT5
Synonyms
Histone acetyltransferase KAT5, 60 kDa Tat-interactive protein, Lysine acetyltransferase 5, cPLA(2)-interacting protein, HTATIP, TIP60
Scientific Information
Q92993 (http://www.uniprot.org/uniprot/Q92993)
Assay Properties
Substrates
Histone H2A, 1.25 μM
Acetyl CoA, 1.5 μM
Reaction
[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| WM-8014 | 8,900 |
| WM-3835 | 4,600 |
| PF-9363 | >10,000 |
| WM-1119 | >10,000 |
| Anacardic Acid | 30,000 |
| Garcinol | 11,000 |
| Curcumin | 25,000 |
| C646 | 21,500 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Histone Acetyltransferase Assay (HAT) Services for Drug Discovery
Reference Compound IC50 for KAT5
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