Activity-based KAT6A/MYST3 Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.
Target Overview
CAT#
KAT6A
Enzyme
Human KAT6A
Synonyms
Histone acetyltransferase KAT6A, MOZ, MYST3, RUNXBP2, ZNF220
Scientific Information
Q92794 (https://www.uniprot.org/uniprotkb/Q92794/)
Assay Properties
Substrates
Histone H3, 5 μM
Acetyl CoA, 0.5 μM
Reaction
[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| WM-8014 | 21 |
| WM-3835 | 4.6 |
| PF-9363 | 1.6 |
| WM-1119 | 12.6 |
| Anacardic Acid | 40,000 |
| Garcinol | 31,000 |
| Curcumin | >100,000 |
| C646 | 13,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Histone Acetyltransferase Assay (HAT) Services for Drug Discovery
Reference Compound IC50 for KAT6A/MYST3
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