IDH1 (G97D) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Target Overview
CAT#
IDH1 (G97D)
Enzyme
Human IDH1 (G97D) Mutant
Synonym
Isocitrate dehydrogenase [NADP] cytoplasmic, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
Scientific Information
O75874 (http://www.uniprot.org/uniprot/O75874)
Assay Properties
Substrate
a-ketoglutarate, 400 µM
NADPH, 15 µM
Reaction
aKG + NADPH = 2HG + NADP
Detection
Resorufin fluorescence (diaphorase/resazurin coupled reaction)
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| AG-120 | 514 |
| AGI-5198 | 16000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Isocitrate Dehydrogenase Assay Services for Drug Discovery.
Reference compound IC50 for IDH1 (G97D)
