IDH1 (R132H) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Target Overview
CAT#
IDH1 (R132H)
Enzyme
Human IDH1 (R132H) mutant
Synonym
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42), NADP(+)-specific ICDH, Oxalosuccinate decarboxylase)
Substrate
1.5 mM α-ketoglutarate, 15 µM NADPH
Assay Properties
Reaction
aKG + NADPH = 2HG + NADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| AGI-5198 | 3.4 |
| IDH-C227 | 7.5 |
| AG-120 | 3.8 |
Scientific Information
O75874 (http://www.uniprot.org/uniprot/O75874)
Screening Location
Malvern, USA
Reference compound IC50 for IDH1 (R132H)
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