IDH1 WT Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Target Overview
CAT#
IDH1 WT
Enzyme
Human IDH1 wild type
Synonym
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42), NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
Scientific Information
O75874 (http://www.uniprot.org/uniprot/O75874 )
Assay Properties
Substrate
Isocitrate, 65 µM
NADP, 50 µM
Reaction
Isocitrate + NADP = aKG + NADPH
Detection
Resorufin fluorescence (diaphorase/resazurin coupled reaction)
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| AG-120 | 8300 |
| AGI-5198 | >20000 |
| IDH-C227 | >20000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Isocitrate Dehydrogenase Assay Services for Drug Discovery.
Reference compound inhibition of IDH1 WT
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