IDH2 WT Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Target Overview
CAT#
IDH2 WT
Enzyme
Human IDH2 wild type
Synonym
Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42); ICD-M; Oxalosuccinate decarboxylase
Scientific Information
P48735 (http://www.uniprot.org/uniprot/P48735)
Assay Properties
Substrate
150 µM isocitrate , 25 µM NADP
Reaction
isocitrate + NADP = a-KG + NADPH
Detection
Resorufin fluorescence (diaphorase/resazurin coupled reaction)
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IDH-C100 | 1800 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Isocitrate Dehydrogenase Assay Services for Drug Discovery.
Reference compound IC50 for IDH2 WT
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