VHL binding assay for compound screening and profiling via probe displacement monitored through a change in fluorescence polarization
Target Overview
CAT#
VHL Direct
Enzyme
Human VHL/EloB/EloC complex
Probe
FAM-DEALAHypYIPMDDDFQLRSF
Scientific Information
P40337 (https://www.uniprot.org/uniprotkb/P40337/entry)
Assay Properties
Reaction
VHL + probe = FP signal
Readout
Change in FP signal upon probe displacement
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| MZ1 | 540.2 |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Protein Degradation Assay – PROTAC Screening
Reference compound inhibition of probe binding to VHL
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