DUSP22/MKPX Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
DUSP22/MKPX
Enzyme
Human DUSP22/MKPX
Synonyms
Dual specificity protein phosphatase 22, JNK-stimulatory phosphatase-1, Low molecular weight dualspecificity phosphatase 2, Mitogen-activated protein kinase phosphatase x, JSP1, LMWDSP2
Substrates
Fluorogenic substrate DiFMUP, 10 μM
Assay Properties
Reaction
Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate[a protein]-serine/threonine phosphate + H₂O = [a protein]-serine/threonine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| NSC-87877 | 7,500 |
| PHPS1 | 580 |
| PTP1B Inhibitor | 13,000 |
Scientific Information
Q9NRW4 (http://www.uniprot.org/uniprot/Q9NRW4)
Screening Location
Malvern, USA
Reference Compound IC50 for DUSP22/MKPX
PDF