PPAC (ACP1/LMW-PTP-A) Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PPAC (ACP1/LMW-PTP-A)
Enzyme
Human PPAC (ACP1/LMW-PTP-A), isoform A
Synonyms
Low molecular weight phosphotyrosine protein phosphatase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, LMPTP-A, HCPTP-A
Substrates
Fluorogenic substrate DiFMUP, 10 μM
Assay Properties
Reaction
Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (μM) |
|---|---|
| PTP1B Inhibitor | 7.4 |
| LMWPTP Inhibitor-1 | 3 |
Scientific Information
P24666 (P24666-1) (http://www.uniprot.org/uniprot/P24666)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Phosphatase Assay Services for Drug Discovery
Reference Compound IC50 for PPAC (ACP1/LMW-PTP-A)
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