PTPN1/PTP1B Catalytic Domain Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PTPN1/PTP1B-CD
Enzyme
Human PTPN1/ PTP1B (Catalytic Domain)
Synonyms
Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B
Substrates
Fluorogenic substrate DiFMUP, 2 μM
Assay Properties
Reaction
Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PTP1B Inhibitor | 3,432 |
| PHPS1 | 430 |
| NSC-87877 | 5,805 |
Scientific Information
P18031 (http://www.uniprot.org/uniprot/P18031)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Phosphatase Assay Services for Drug Discovery
Reference Compound IC50 for PTPN1/PTP1B-CD
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