Factor IXa Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Factor IXa
Enzyme
Coagulation factor IX
Synonym
Coagulation factor IX, Plasma thromboplastin component
Substrates
Fluorogenic substrate Mca-RPKPVE-Nval-WRK[Dnp]-NH2, 5 µM
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Nafamostat Mesylate | 0.04 |
| Gabexate Mesylate | 48.1 |
| Leupeptin | >100 |
Scientific Information
P00740 (http://www.uniprot.org/uniprot/P00740)
Screening Location
Malvern, USA
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