MMP 7 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP7
Enzyme
MMP-7, catalytic domain from human recombinant (residues 78-250) expressed in E. coli with C-terminal tag
Synonym
Pump-1 protease, Uterine metalloproteinase, Matrix metalloproteinase-7, MMP-7, Matrin
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 4.40E-06 |
| Actinonin | 2.33E-06 |
| GM6001 | 3.66E-09 |
| MMP Inhibitor III | 1.90E-08 |
| MMP-9/MMP-13 Inhibitor I | 1.61E-06 |
Scientific Information
P09237 (http://www.uniprot.org/uniprot/P09237)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-7
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