PDE10A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE10A
Enzyme
Human PDE10A
Synonym
cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A
Substrate
1 µM cAMP
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 12,000 |
| Methoxymethyl-IBMX | 29,000 |
| Roliptam | >50,000 |
| TC-E 5005 | 10 |
Scientific Information
Q9Y233 (http://www.uniprot.org/uniprot/Q9Y233)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE10A
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