PDE5A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE5A
Enzyme
Human PDE5A
Synonym
cGMP-specific 3′,5′-cyclic phosphodiesterase, CGB-PDE)
Substrate
1 µM cGMP
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
IBMX | >50,000 |
Methoxymethyl-IBMX | >50,000 |
Roliptam | >50,000 |
Methoxyquinazoline | 7,900 |
Scientific Information
O76074 (http://www.uniprot.org/uniprot/O76074)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE5A
![Reference Compound IC50 for PDE5A](https://www.reactionbiology.com/wp-content/uploads/2023/11/PDE5A_Malvern.png?itok=Z3vXCIPo)
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