PDE5A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE5A
Enzyme
Human PDE5A
Synonym
cGMP-specific 3′,5′-cyclic phosphodiesterase, CGB-PDE)
Substrate
1 µM cGMP
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | >50,000 |
| Methoxymethyl-IBMX | >50,000 |
| Roliptam | >50,000 |
| Methoxyquinazoline | 7,900 |
Scientific Information
O76074 (http://www.uniprot.org/uniprot/O76074)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE5A
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