PDE9A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE9A
Enzyme
Human PDE9A
Synonym
High affinity cGMP-specific 3′,5′-cyclic phosphodiesterase 9A
Substrate
0.2 µM cGMP
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | >100,000 |
| Methoxymethyl-IBMX | >100,000 |
| Roliptam | >100,000 |
| Zaprinast | 10,500 |
Scientific Information
O76083 (http://www.uniprot.org/uniprot/O76083)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE9A
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