UCHL1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
UCHL1
Enzyme
Human UCHL1
Synonym
Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, UCH-L1)
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Ub Aldehyde | 17.8 |
| UCHL1 I 2 | 16.7 |
| UCHL3 I | 88.3 |
Scientific Information
P09936 (http://www.uniprot.org/uniprot/P09936)
Screening Location
Malvern, USA
Reference Compound IC50 for UCHL1
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