UCHL3 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
UCHL3
Enzyme
Human UCHL3
Synonym
Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, UCH-L3
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 6.26 |
| UIPase I | >200 |
| UI1 | 157 |
Scientific Information
P15374 (http://www.uniprot.org/uniprot/P15374)
Screening Location
Malvern, USA
Reference compound IC50 for UCHL3
PDF