USP2 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP2
Enzyme
Human USP2
Synonym
Ubiquitin carboxyl-terminal hydrolase 2, 41 kDa ubiquitin-specific protease, Deubiquitinating enzyme 2, Ubiquitin thiolesterase 2, Ubiquitin-specific-processing protease 2, UBP41, UBP2
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 23.2 |
| UIPase I | 165 |
| UI1 | >200 |
Scientific Information
O75604 (http://www.uniprot.org/uniprot/O75604)
Screening Location
Malvern, USA
Reference Compound IC50 for USP2
PDF