USP8 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP8
Enzyme
Human USP8
Synonym
Ubiquitin carboxyl-terminal hydrolase 8, Deubiquitinating enzyme 8, Ubiquitin isopeptidase Y, Ubiquitin thiolesterase 8, Ubiquitin-specific-processing protease 8, KIAA0055, UBPY, UBP8
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 8.3 |
| UIPase I | >200 |
| UI1 | 72.9 |
Scientific Information
P40818 (http://www.uniprot.org/uniprot/P40818)
Screening Location
Malvern, USA
Reference Compound IC50 for USP8
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