USP7 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP7
Enzyme
Human USP7
Synonym
HAUSP, Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7
Substrate
Ubiquitin Rhodamine 110 Protein, 100 nM
Assay Properties
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| FT671 | 5.06 |
| UCH-L1 Inhibitor | 5,677 |
| UCH-L3 Inhibitor | 5,520 |
Scientific Information
Q93009 (https://www.uniprot.org/uniprotkb/Q93009/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP7
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